NASC

NASC ID: N849338

Name: WiscDsLox233237_19L

ABRC stock number: CS849338

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox233237_19L.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G13840.1-P355 and AT4G13850.2-P355 (chr 4 pos 8019384 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796035.

Wiscseq_DsLox233237_19L.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G30200.4-P355 and AT4G30210.1-P355 (chr 4 pos 14791027 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796033.

Wiscseq_DsLox233237_19L.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G30310.1 (chr 4 pos 14834397 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796034.

Wiscseq_DsLox233237_19L.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64560.1 (at chr 5 pos 25808147 (C/25808147-25808216) on the TAIR10). The sequences' 1-70 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 0, R 3, C 2 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453583.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox233237_19L

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G13840 AT4G13850 AT4G30200 AT4G30210 AT4G30310 AT5G64560